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MAB4301

Sigma-Aldrich

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

clone MC-480, Chemicon®, from mouse

Synonym(s):
SSEA-1, CD15, Lewis X, 3-FAL
eCl@ss:
32160702

Quality Level

biological source

mouse

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

MC-480, monoclonal

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

input

sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... FUT4(2526)

Related Categories

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Immunogen

F9 tetracarcinoma stem cells (X-irradiated).

Application

Research Sub Category
Pluripotent & Early Differentiation

Neural Stem Cells
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research

Quality

Tested

Target description

220 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Ammonium Sulfate Precipitation

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
P19 mouse embryonic carcinoma cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

The efficient generation of cell lines from bovine parthenotes.
Pashaiasl M, Khodadadi K, Holland MK, Verma PJ
Cellular Reprogramming null
Z Cao et al.
The Journal of biological chemistry, 276(24), 21714-21723 (2001-03-30)
In this study we demonstrate that in corneal epithelium there is cell-cell contact-regulated expression of a 145-kDa glycoprotein (GP) bearing the glycan determinant Lewis(x) (Le(x)) (Galbeta(1,4)[Fucalpha(1,3)]GlcNAc). This glycoprotein (Le(x)-GP) was expressed in confluent/contact-inhibited cultures but not in sparse cultures of
Yan-Min Feng et al.
Cell cycle (Georgetown, Tex.), 13(5), 782-791 (2014-01-09)
A critical process of early oogenesis is the entry of mitotic oogonia into meiosis, a cell cycle switch regulated by a complex gene regulatory network. Although Notch pathway is involved in numerous important aspects of oogenesis in invertebrate species, whether
Induced pluripotent reprogramming from promiscuous human stemness related factors.
Nelson TJ, Martinez-Fernandez A, Yamada S, Mael AA, Terzic A, Ikeda Y
Clinical and Translational Science null
Yueh-Chiang Hu et al.
PLoS genetics, 9(7), e1003629-e1003629 (2013-07-23)
In mammals, both testis and ovary arise from a sexually undifferentiated precursor, the genital ridge, which first appears during mid-gestation as a thickening of the coelomic epithelium on the ventromedial surface of the mesonephros. At least four genes (Lhx9, Sf1

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