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MAB5352

Sigma-Aldrich

Anti-Notch 1 Antibody, clone mN1A

clone mN1A, Chemicon®, from mouse

eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mN1A, monoclonal

species reactivity

feline, mouse, rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... NOTCH1(4851)

Related Categories

General description

Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).

Specificity

Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.

Immunogen

Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.

Application

Research Sub Category
Transcription Factors
Western blot using ECL

Immunohistochemistry

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by the end user.
Anti-Notch 1 Antibody, clone mN1A is a high quality Mouse Monoclonal Antibody for the detection of Notch 1 & has been validated in ICC, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function

Target description

272.5 kDa (also 230 kDa processed form)

Linkage

Replaces: 04-1046

Physical form

Format: Purified
Purified immunoglobulin. Liquid.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Shu Li et al.
Molecular medicine reports, 9(6), 2233-2238 (2014-03-20)
Brain arteriovenous malformation (bAVM) is currently one of the most common cerebral vascular diseases, which result in severe clinical outcomes. The Notch signaling pathway is involved in vasculogenesis and angiogenesis, as well as vascular remodeling and arteriovenous differentiation in multiple
Huan-You Wang et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 23(6), 773-780 (2010-03-30)
Primary effusion lymphoma is a human herpes virus 8 (HHV-8)-associated large cell lymphoma of body cavities. Detailed large-scale clinicopathological studies are rarely reported, and the underlying mechanism of lymphomagenesis remains elusive. In the present report, we studied the clinicodemographic, immunophenotypic
David F Fischer et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(11), 1451-1458 (2005-08-30)
Down syndrome (DS) patients suffer from mental retardation, but also display enhanced beta-APP production and develop cortical amyloid plaques at an early age. As beta-APP and Notch are both processed by gamma-secretase, we analyzed expression of the Notch signaling pathway
Susanne Pettersson et al.
The Biochemical journal, 450(3), 523-536 (2012-12-21)
The Notch receptor is necessary for modulating cell fate decisions throughout development, and aberrant activation of Notch signalling has been associated with many diseases, including tumorigenesis. The E3 ligase MDM2 (murine double minute 2) plays a role in regulating the
MDM2 protein-mediated ubiquitination of numb protein: identification of a second physiological substrate of MDM2 that employs a dual-site docking mechanism.
Sczaniecka, M; Gladstone, K; Pettersson, S; McLaren, L; Huart, AS; Wallace, M
The Journal of Biological Chemistry null

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