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Merck
CN

MAB805

Anti-Adenovirus (Blend) Coating Antibody, clone 2/6, and 20/11

ascites fluid, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
2/6, monoclonal, 20/11, monoclonal
Species reactivity:
human
Application:
ELISA, IF, IHC (p)
Citations:
47
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biological source

mouse

Quality Level

antibody form

ascites fluid

clone

2/6, monoclonal, 20/11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1κ

shipped in

wet ice

storage temp.

−20°C

General description

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Immunogen

Adeno 3

Application

Research Sub Category
Infectious Diseases - Viral
Research Category
Infectious Diseases
Anti-Adenovirus (Blend) Coating Antibody, clone 2/6 & 20/11 is an antibody against Adenovirus (Blend) Coating for use in ELISA, IF, IH, IH(P).
EIA: 1:1000

IFA: 1:200

Immunohistochemistry: 1:400 - 1:1000 for formalin fixed paraffin-embedded or frozen tissue.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reactive with all 41 serotypes of Adenovirus.

Physical form

Ascites fluid containing no preservatives.
Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Adenovirus Control Slides, Catalogue Number 5009-5

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Silencing E1A mRNA by RNA interference inhibits adenovirus replication.
Y-S Chung,M-K Kim,W-J Lee,C Kang
Archives of Virology null
R Alemany et al.
Methods in molecular medicine, 35, 395-412 (2000-01-01)
It is important to analyze to what extent these random or designed mutations abrogate viral replication in normal cells because a tightly controlled vector could be injected at higher doses intratumorally or even systemically. On the other hand, it is
Yue-Hong Shen et al.
PloS one, 11(1), e0147173-e0147173 (2016-01-23)
CAR is a transmembrane protein that is expressed in various epithelial and endothelial cells. CAR mediates adenoviral infection, as well as adenovirus-mediated oncolysis of AxdAdB-3, an E1A/E1B double-restricted oncolytic adenovirus, in prostate cancer cells. This study further assessed the therapeutic



Global Trade Item Number

SKUGTIN
MAB80504053252465307