Anti-N-Myc Interactor Antibody, clone 16E9.2

N-myc-interactor (UniProt Q13287; also known as N-myc and STAT interactor, Nmi) is encoded by the NMI gene (Gene ID 9111) in human. Nmi is expressed in all adult and fetal tissues except brain and skin and is shown to be more abundant in fetal liver. It interacts with all STATs, except STAT2 and augments STAT-mediated transcription in response to IL-2 and gamma-interferon. Interestingly, Nmi lacks an intrinsic transcriptional activation domain; instead, it enhances the association of CBP/p300 coactivator proteins with STAT1 and STAT5. Higher expression of Nmi has been reported in myeloid leukemia that also express higher levels of c-Myc. Ref.: Zhu, M., et al. (1999). Cell 96, 121-130.

~39 kDa observed. 35.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinant human N-Myc Interactor N-terminal fragment.

Detect N-Myc Interactor using this mouse monoclonal Anti-N-Myc Interactor Antibody, clone 16E9.2, Cat. No. MABC1235, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Myc Interactor in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected N-Myc Interactor in human cerebellum, small instestine, and tonsil tissue sections.

Research Category
Apoptosis & Cancer

Clone 16E9.2 targeted epitope resides within the N-terminal half of N-Myc Interactor.

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected N-Myc Interactor in 10 µg of K562 cell lysate.

Concentration: Please refer to lot specific datasheet.

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