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MABC154

Sigma-Aldrich

Anti-RRM2 Antibody, clone 4D1.2

clone 4D1.2, from mouse

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Synonym(s):
Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4D1.2, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001025

UniProt accession no.

P31350

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RRM2(6241)

General description

Ribonucleoside-diphosphate reductase subunit M2 (RRM2) is a cytoplasmic protein, which catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, and plays an important role in DNA damage and repair. RRM2 has been implicated in various types of cancer.

Immunogen

GST-tagged recombinant protein corresponding to human RRM2.

Application

Detect Ribonucleotide Reductase using this mouse monoclonal antibody, Anti-RRM2 Antibody, clone 4D1.2 validated for use in western blotting & ICC.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RRM2 in HepG2 and A431 cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected RRM2 in 10 µg of HeLa cell lysate.

Target description

~45 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Michal W Luczak et al.
Cell cycle (Georgetown, Tex.), 20(18), 1812-1827 (2021-08-13)
The hypoxia-induced transcription factor HIF1 inhibits cell growth in normoxia through poorly understood mechanisms. A constitutive upregulation of hypoxia response is associated with increased malignancy, indicating a loss of antiproliferative effects of HIF1 in cancer cells. To understand these differences

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