Anti-Bax Antibody, clone 3C10

Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. Bax displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Bax is largely cytosolic under normal conditions, but upon induction of apoptosis it undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. Bax is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 3C10 that is specific to the Bax 1- 2 loop activates mitochondrial Bax, but blocks translocation of cytosolic Bax. It recognizes a peptide sequence at the start of Bax 1 2 loop and can bind mitochondrial Bax to induce dimerization and cytochrome c release.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Bax in Immunoprecipitation applications

Inhibits Activity/Function Analysis: A representative lot prevented tBid-triggered Bax translocation and cytochrome c release

Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications

nduces Function Analysis: A representative lot of this antibody activated mitochondrial Bax

Evaluated by Western Blotting in wild type (WT) HeLa cells and Bax/Bak double knockout HeLa cells.

Purified rat monoclonal antibody IgG2a in PBS without azide.

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