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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
antibody form
purified antibody
clone
Kt-IgM-8
monoclonal
purified by
(Ligatrap)
species reactivity
human, human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgM
suitability
suitable for immunoassay
General description
Semi-synthetic Tn-PS A1 (Tn= D-GalpNAc)
Application
Affects Function Analysis: A representative lot showed high complement-dependent cytotoxicity (CDC) activity. (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
Flow Cytometry Analysis: A representative lot detected Tn-antigen in Flow Cytometry applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
Enzyme Immunoassay Analysis: A representative lot detected Tn-antigen in ELISA applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
Flow Cytometry Analysis: A representative lot detected Tn-antigen in Flow Cytometry applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
Enzyme Immunoassay Analysis: A representative lot detected Tn-antigen in ELISA applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
Biochem/physiol Actions
Clone KT-IgM-8 is a mouse monoclonal antibody that detects Thomsen-nouveau (Tn) polysaccharide A1 monovalently, including in human tumor cells.
Physical form
Purified mouse monoclonal antibody IgM in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Flow Cytometry in MCF-7 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Tn-antigen in one million MCF-7 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Tn-antigen in one million MCF-7 cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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