Anti-BRMS1, clone 3a1.21, Cat. No. MABC1753, is a mouse monoclonal antibody that detects Breast cancer metastasis-suppressor 1 and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Immunoprecipitation Western Blotting

Immunohistochemistry Analysis: A representative lot detected BRMS1 in Immunohistochemistry applications (Slipicevic, A., et. al. (2012). BMC Cancer. 12:73; Zhang, G., et. al. (2012). PLoS One. 7(9):e45037).

Immunoprecipitation Analysis: A representative lot immunoprecipitated BRMS1 in Immunoprecipitation applications (Hurst, D.R., et. al. (2006). Biochem Biophys Res Commun. 348(4):1429-35).

Western Blotting Analysis: A representative lot detected BRMS1 in Western Blotting applications (Hurst, D.R., et. al. (2006). Biochem Biophys Res Commun. 348(4):1429-35).

Clone 3a1.21 is a mouse monoclonal antibody that detects human Breast cancer metastasis-suppressor 1. It targets an epitope within the first 50 amino acids from the N-terminal region.

GST-tagged full-length recombinant human Breast cancer metastasis-suppressor 1.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Breast cancer metastasis-suppressor 1 (UniProt: Q9HCU9, also known as BRMS1) is encoded by the BRMS1 gene (Gene ID: 25855) in human. BRMS1 is an homohexameric, predominantly nuclear protein with two nuclear localization sequences (aa 198-205 and 238-244) but may also have a cytoplasmic role. Its cytoplasmic expression has been linked to higher disease-free survival in cases of malignant melanoma. It is a member of the mSin3-HDAC transcription co-repressor complex. It is reported to suppress metastasis in multiple human and murine carcinoma cell lines by altering the expression of both coding and non-coding metastasis associated genes. It down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RelA at lysine 310 and HDAC1 binding to promoter regions. It is also shown to down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. HSP90 is reported to stabilize the levels of BRMS1 in cells, which may be important in maintaining the functional role of BRMS1 as a metastasis suppression. (Ref.: Hurst, DR et al., PLoS One. 2013;8(2); e55966; Hurst, DR et al. (2006). Biochem. Biophys. Res. Commun. 348(4); 1429- 1435; Slipicevic, A., et al. (2012). BMC Cancer 12; 73).

Evaluated by Western Blotting in HepG2 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BRMS1 in HepG2 cell lysates.

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