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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FE9, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... APC(324)
General description
Adenomatous polyposis coli protein (APC) is a ubiquitous multidomain protein that has a well documented role as a tumor suppressor. The mechanism of APC-mediated tumor suppression is still an area of active investigation; however, several studies suggest that APC is a negative regulator of the Wnt signaling pathway as it downregulates β-catenin via interactions with Axin/GSK3-β complex, thereby preventing transcription of genes such as MYC that contribute to cell proliferation. Defective APC proteins contribute to the initiation and proliferation of various types of cancers, including familial adenomatous polyposis. However, other studies have shown that APC interacts with a range of other proteins such as the EB1 microtubule-binding proteins, to regulate other processes such as cell migration.
~147 kDa observed. Uniprot describes the full length protein at ~312 kDa This antibody was shown to detect the truncated form at ~147 kDa
Specificity
This antibody recognizes the N-terminus of APC.
Immunogen
Linear peptide corresponding to the N-terminus of human APC.
Application
Anti-APC Antibody, clone FE9 is an antibody against APC for use in Western Blotting.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in SW480 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected APC in 10 µg of SW480 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected APC in 10 µg of SW480 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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