Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.

~65 kDa observed.
The calculated molecular weight of this protein is 41 kDa, but has been observed at ~65-90 kDa due to glycosolation (de Saint-Vis, B., et al. (1998). Immunity. 9(3):325-336).

Recombinant protein corresponding to human LAMP-3.

Detect LAMP-3 using this Anti-LAMP-3 Antibody, clone 16H11.2 validated for use in WB, IH.

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected LAMP-3 in colorectal adenocarcinoma tissue.

Research Category
Cell Structure

Research Sub Category
Apoptosis - Additional

Organelle & Cell Markers

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
MCF-7 cell lysate

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-3 in MCF-7 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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