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Merck
CN

MABE215

Sigma-Aldrich

Anti-Liver receptor homolog-1 Antibody, clone 1LRH-2E1

ascites fluid, clone 1LRH-2E1, from mouse

Synonym(s):

Nuclear receptor subfamily 5 group A member 2, Liver receptor homolog 1, LRH-1, NR5A2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1LRH-2E1, monoclonal

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_109601

UniProt accession no.

P45448

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Nr5A2(26424)

General description

Liver receptor homolog 1 (LRH-1, CPF, FTF, PHR-1, FTF, or hB1F) is an orphan nuclear receptor that is highly expressed in the intestines, pancreas, ovary, and liver. LRH-1 is a constitutively active transcription factor with a complex role in many cellular processes which may include lipid metabolism, cell proliferation, development, reproduction, and immunity. LRH-1 may interact with an array of transcriptional coactivators that modulate its activity, among which are the MBF1, SRC, and PGC-1α proteins. In addition, ERK phosphorylation on Ser-238 and Ser-243 also enhances LRH-1 activity. However, LRH-1 is also negatively regulated by several factors. For instance, corepressors such as SHP, DAX-1, and PROX-1, preclude its binding to DNA; and sumoylation promotes its inactivation and sequestration into nuclear bodies. LRH-1 targets many genes including the CYP7A1 and CYP8B1 genes involved in bile synthesis; and Oct4 required for pluripotency in embryonic stem cells. LRH-1 has been implicated in inflammatory bowel disease, and tumor development in the intestines and pancreas.
~64 kDa, ~56 kDa, and ~42 kDa observed. This antibody detects three isoforms of Liver receptor homolog-1 as described by Uniprot No. O00482.

Immunogen

Linear peptide corresponding to the mouse Liver receptor homolog-1.

Application

Use Anti-LRH-1 Antibody, clone 1LRH-2E1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect LRH-1 also known as Liver receptor homolog 1.
Western Blot Analysis: A 1:1,000 dilution from representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cell lysates (M. Oulad, IGBMC, France.).

Immunocytochemistry Analysis: A representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cells (M. Oulad, IGBMC, France.).

Physical form

Mouse monoclonal IgG2aλ ascites with 0.05% sodium azide.

Analysis Note

Control
Mouse pancreas tissue lysate
Evaluated by Western Blot in mouse pancreas tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Liver receptor homolog-1 in 10 µg of mouse pancrease tissue lysate.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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