Anti-acetyl PARP1 Antibody, clone E4

Poly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.

~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.

Linear peptide corresponding to acetylated human PARP1, short fragment.

Anti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Acetylated PARP1 fragment (373-529) purified protein lysate

Evaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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