MABE60
Anti-BRG1 Antibody, clone 3G4
clone 3G4, from rat
Synonym(s):
Transcription activator BRG1, ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3G4, monoclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SMARCA4(6597)
General description
Transcription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.
~185 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human BRG1.
Application
Anti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Transcription Factors
Chromatin Biology
Transcription Factors
Western Blotting Analysis: A representative lot detected BRG1 (SMARCA4) in THP-1 human leukemia monocytic cell lysates (Cruickshank, V.A., et al. (2015). PLoS One. 10(11):e0142806).
Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).
Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Che-Chia Hsu et al.
The Journal of biological chemistry, 287(27), 22533-22548 (2012-05-09)
The nucleolar 58-kDa microspherule protein (MSP58) protein is a candidate oncogene implicated in modulating cellular proliferation and malignant transformation. In this study, we show that knocking down MSP58 expression caused aneuploidy and led to apoptosis, whereas ectopic expression of MSP58
Vural Tagal et al.
Nature communications, 8, 14098-14098 (2017-01-20)
Mutations in the SMARCA4/BRG1 gene resulting in complete loss of its protein (BRG1) occur frequently in non-small cell lung cancer (NSCLC) cells. Currently, no single therapeutic agent has been identified as synthetically lethal with SMARCA4/BRG1 loss. We identify AURKA activity
Manjeet K Rao et al.
The Journal of biological chemistry, 289(51), 35087-35101 (2014-10-22)
Genome-wide studies have revealed that genes commonly have a high density of RNA polymerase II just downstream of the transcription start site. This has raised the possibility that genes are commonly regulated by transcriptional elongation, but this remains largely untested
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