Mitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.

~83 kDa observed

His-tagged recombinant protein corresponding to human Mad1.

Anti-Mad1 Antibody, clone BB3-8 is a highly specific mouse monoclonal antibody, that targets MAD1 & has been tested in western blotting & Immunofluorescence.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).

Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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