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Merck
CN

MABF116

Anti-CD3 gamma delta (HMT3-2) Antibody, clone Ham25-1157

clone Ham25-1157, from hamster

Synonym(s):

T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD3g, HMT3-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
Ham25-1157, monoclonal
Species reactivity:
human
Application:
FACS
Citations:
-

Key Documents

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biological source

hamster

antibody form

purified antibody

antibody product type

primary antibodies

clone

Ham25-1157, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

flow cytometry: suitable

NCBI accession no.

NP_033980

UniProt accession no.

P11942

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD3G(917)

General description

21 kDa calculated
The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains: gamma, delta, epsilon and zeta. CD3 is first detectable in early thymocytes and one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocytes, it is detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies.

Immunogen

Purified protein corresponding to mouse CD3 gamma delta.

Application

Anti-CD3 gamma delta (HMT3-2) Antibody, clone Ham25-1157 detects level of CD3 gamma delta (HMT3-2) & has been published & validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

This antibody recognizes CD3 gamma delta.

Physical form

Format: Purified
Protein G Purified
Purified armenian hamster monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cells
Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: 2.5 µg/mL of this antibody detected CD3 gamma delta in 1X10E5 Jurkat cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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