Anti-CD69 Antibody, clone FN61

22.56 kDa calculated.

Activated B cells from human peripheral blood.

Epitope: extracellular domain

Clone FN61 is a mouse monoclonal antibody that detects human Early activation antigen CD69. It targets an epitope within the E1 site in the extracellular domain.

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Flow Cytometry in Raji cells pretreated with 50 ng/mL PMA and 1 ug/mL ionomycin.

Flow Cytometry Analysis: 1 µg of this antibody detected CD69 in one million Raji cells pretreated with Phorbol 12-myristate 13-acetate (PMA; 50 ng/mL) and Ionomycin (1 ug/mL) for 4 to 6 hours.

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