MABF2233
Anti-West Nile Virus Antibody, clone E60
Sign Into View Organizational & Contract Pricing
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
E60
monoclonal
purified by
using protein G
species reactivity
virus, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
capacity
neutralization
UniProt accession no.
target post-translational modification
unmodified
General description
West Nile Virus envelope protein.
~47 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Johnson, B., et. al. (2018). Immunity. 48(3):487-499).
Neutralizing Analysis: A representative lot partially neutralized West Nile Virus infection. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Neutralizing Analysis: A representative lot partially neutralized West Nile Virus infection. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Biochem/physiol Actions
Clone E60 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DII fusion loop.
Physical form
Purified mouse monoclonal antibody IgG2a in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting with full-length ectodomain of West nile virus envelope protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected full-length ectodomain of West nile virus envelope protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected full-length ectodomain of West nile virus envelope protein.
Other Notes
Virus (West Nile Virus).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service