MABF2264
Anti-IRGM1 (LRG-47) Antibody, clone 1B2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
1B2
monoclonal
purified by
using protein G
species reactivity
mouse, mouse, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
46.55 kDa calculated.
A linear peptide corresponding to 15 amino acids from the N-terminal region of mouse Immunity-related GTPase family M protein 1 (IRGM1).
Application
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in Western Blotting applications (Butcher, B.A., et. al. (2005). Infect Immun. 73(6):3278-86; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in wild-type, but not in Irgm1 KO NIH3T3 cells treated with IFN-gamma (Courtesy of Brian Fee, Ph.D. and Gregory A. Taylor, Ph.D., Duke University School of Medicine, USA).
Immunofluorescence Analysis: A representative lot detected IRGM1 (LRG-47) in Immunofluorescence applications (Zhao, Y.O., et. al. (2010). PLoS One. 5(1):e8648; Henry, S.C., et. al. (2014). PLoS One. 9(4):e95021; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
Western Blotting Analysis: A representative lot detected IRGM1 (LRG-47) in wild-type, but not in Irgm1 KO NIH3T3 cells treated with IFN-gamma (Courtesy of Brian Fee, Ph.D. and Gregory A. Taylor, Ph.D., Duke University School of Medicine, USA).
Immunofluorescence Analysis: A representative lot detected IRGM1 (LRG-47) in Immunofluorescence applications (Zhao, Y.O., et. al. (2010). PLoS One. 5(1):e8648; Henry, S.C., et. al. (2014). PLoS One. 9(4):e95021; Springer, H.M., et. al. (2013). J Immunol. 191(4):1765-74).
Biochem/physiol Actions
Clone 1B2 is a mouse monoclonal antibody that detects the long form of Immunity-related GTPase family M protein 1 (IRGM1). It targets an epitope within 15 amino acids from the N-terminal region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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