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Merck
CN

MABF2317

Sigma-Aldrich

Anti-CD23 Antibody, clone EBVCS1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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conjugate

unconjugated

antibody form

purified antibody

clone

EBVCS1
monoclonal

purified by

using protein G

species reactivity

human, human, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

target post-translational modification

unmodified

General description

Epstein-Barr Virus transformed human cells.
~45 kDa observed; 36.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Immunofluorescence Analysis: A representative lot detected CD23 in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).

Radioimmunoassay Analysis: A representative lot detected CD23 in Radioimmunoassay applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD23 in Immunoprecipitation applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60; Sugden, B., et. al. (1983). J Virol. 46(3):800-7).

Flow Cytometry Analysis: A representative lot detected CD23 in Flow Cytometry applications (Palaniyandi, S., et. al. (2011). J Immunol. 186(6):3484-96).

Immunocytochemistry Analysis: A representative lot detected CD23 in Immunocytochemistry applications (Palaniyandi, S., et. al. (2011). J Immunol. 186(6):3484-96).

Western Blotting Analysis: A representative lot detected CD23 in Western Blotting applications (Palaniyandi, S., et. al. (2011). J Immunol. 186(6):3484-96).

Biochem/physiol Actions

Clone EBVCS1 is a mouse monoclonal antibody that specifically detects CD23 on Epstein-Barr virus transformed cells.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in U937 cell lysates.

Western Blotting Analysis: 4 µg/mL of this antibody detected CD23 in U937 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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