MABF2340
Anti-CD300a (CLM-8) Antibody, clone TX41
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
TX41
monoclonal
purified by
using protein G
species reactivity
rat, mouse, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG2aκ
suitability
suitable for immunoassay
UniProt accession no.
target post-translational modification
unmodified
General description
Ba/F3 cells transfected with murine CLM-8 (MAIR-I/CD300a) fused with the Fc portion of human IgG.
~ 30 kDa observed; 35.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Flow Cytometry Analysis: A representative lot detected CD300a (CLM-8) in Flow Cytometry applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).
Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).
Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).
Biochem/physiol Actions
Clone TX41 is a rat monoclonal antibody that specifically detects mouse CD300a (CLM-8).
Physical form
Purified rat monoclonal antibody in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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