Anti-HHV-8 PF-8 Antibody, clone 11D1

Human herpesvirus-8 (also known as Kaposi′s sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. The most prominent protein expressed in latently infected cells is the ORF73 gene product known as the latency-associated nuclear antigen (LANA) that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. The ORF59 (also known as PF-8), a phosphoprotein, is reported to be a processivity factor for HHV-8 DNA polymerase (Pol-8). It interacts with and stimulates Pol-8 DNA synthesis activity. It is an early-late gene product localized to HHV-8-infected cell nuclei. PF-8 interacts with HHV-8 DNA polymerase (Pol-8) and stimulates Pol-8 DNA synthesis activity. Amino acid residues 10-27 and 279-301 are reported to be critical for the processivity function of PF-8. Residues 10 to 27 are essential for its interaction with Pol-8 and 279-301 are required for its binding to dsDNA. Clone 11D1 specifically detects PF-8 of HHV-8 and exhibits predominant nuclear membrane fluorescence in TPA (phorbol ester)-induced, HHV-infected BCBL-1 cells. (Ref.: Strahan, RC., et al. (2017). PLoS Pathog 13(7); e1006482; Chan, SR., and Chandran, B (2000). J. Virol. 74(23); 10920-10929; Chan, SR., et al. (1998). 240: 118-126).

Clone 11D1 is a mouse monoclonal antibody that detects PF-8 of Kaposi′s sarcoma-associated herpesvirus (HHV-8).

12-O-Tetradecanoylphorbol 13-acetate (TPA)-induced BCBL-1 cells.

Quality Control Testing

Evaluated by Immunocytochemistry in BCBL-1 cells.

Immunocytochemistry Analysis (ICC): A 1:50 dilution of this antibody detected Kaposi s sarcoma associated herpesvirus (HHV-8) in 12-O-Tetradecanoylphorbol 13-acetate (TPA) treated (20 ng/mL; 48 h) BCBL-1 cells.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated PF-8 of HHV-8 in Immunoprecipitation applications (Chan, S.R., et al. (1998). Virology. 240(1):118-26; Chan, S.R., et al. (2000). J Virol. 74(23):10920-9).

Western Blotting Analysis: A representative lot detected OF-8 of HHV-8 in Western Blotting applications (Chan, S.R., et al. (1998). Virology.;240(1):118-26; Chan, S.R., et al. (2000). J Virol.;74(23):10920-9).

Immunocytochemistry Analysis: A representative lot detected PF-8 of HHV-8 in Immunocytochemistry applications (Chan, S.R., et al. (1998). Virology.;240(1):118-26).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HHV-8 PF-8, clone 11D1, Cat. No. MABF2752, is a mouse monoclonal antibody that detects PF-8 of Kaposi′s sarcoma-associated herpesvirus (HHV-8) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.