Anti-SARS-CoV-2 ACE2 binding domain Antibody, clone MCA-2G1

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, Peplomer protein) is encoded by the S gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Ravichandran, S., et al. (2020). Sci Transl Med. 12(550):eabc3539).

Recombinant SARS-CoV2 S-Protein ACE2 binding domain from the N-terminal half.

Quality Control Testing

Evaluated by Western Blotting with recombinant His-tagged SARS-CoV-2 spike protein.

Western Blotting Analysis: A 1:100,000 dilution from a representative lot detected recombinant His-tagged SARS-CoV-2 spike protein.

Tested Applications

Immunocytochemistry Analysis: 1:1,000 dilution from a representative lot detected SARS-CoV2 spike protein ACE2 binding domain in HEK293 cells transfected with DNA encoding the S-protein of SARS-CoV2 binding domain.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone MCA-2G1 is a mouse monoclonal antibody that detects SARS-CoV2 S-protein ACE2 binding domain. It targets an epitope within 234 amino acids from the N-terminal half.

Purified mouse monoclonal antibody IgG1 in buffer containing PBS with 5 mM sodium azide with 50% glycerol.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.