Anti-IL-2 (human) Antibody, PE, clone MQ1-17H12

18 kDa calculated

Interleukin-2 (IL-2) is commonly considered a pro-inflammatory cytokine that stimulats T-cell-dependent immune reactions and controls regulatory T cells or Tregs. IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation and other activities crucial to regulation of the immune response. Recent studies have shown that IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Structurally, IL-2 is thought to have a 4-helix-bundle-type fold and uses a novel type I cytokine receptor (IL-21R) with the common gamma chain cytokine receptor. IL-2 also may intensify Th1-mediated diseases, including autoimmune arthritis and may also be an interesting clinical target. IL-2 has also been implicated as a possible therapy for infection and cancer. Anti-tumor therapies that include a combination of recombinant IL-2 and recombinant interferon alpha have been promising in clinical studies. Additionally, a chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL).

E. coli - expressed recombinant human IL-2

Anti-IL-2 (human) Antibody, PE, clone MQ1-17H12 is a highly specific rat monoclonal antibody, that targets IL-2 & has been tested in Flow Cytometry, IHC & IP.

Immunohistochemistry Analysis: A representative lot detected human IL-2 in Immunohistochemistry. (Andersson J, et al. 1994; Fernandez V, et al. 1994; Skansen-Saphir U, et al. 1994; Andersson U, et al. Detection and Quantification of Gene Expression; Raqib R, et al. 2002)

Immunoprecipitation Analysis: A representative lot detected human IL-2 in Immunoprecipitation. (Abrams J, et al. 1992.)

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Affinity

Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 20 μL of this antibody detected IL-2 in one million PMA/ionomycin-stimulated human PBMCs.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.