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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Species reactivity:
human, mouse
Conjugate:
unconjugated
Application:
ELISA, IF, IHC, WB, inhibition assay
Citations:
-
conjugate
unconjugated
antibody form
purified antibody
clone
aB1788G7G6, monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, inhibition assay: suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Alpha(B)-crystallin.
~20 kDa observed; 20.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha B-crystallin in mouse eye and human retina tissue lysate.
Immunohistochemistry Analysis: A representative lot detected alpha B-crystallin in Immunohistochemistry applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
ELISA Analysis: A representative lot detected alpha B-crystallin in ELISA applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Inhibition Analysis: A representative lot inhibited the chaperone and anti-apoptotic activities of alpha B-crystallin. (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Immunofluorescence Analysis: A representative lot detected alpha B-crystallin in Immunofluorescence applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Western Blotting Analysis: A representative lot detected alpha B-crystallin in Western Blotting applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Immunohistochemistry Analysis: A representative lot detected alpha B-crystallin in Immunohistochemistry applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
ELISA Analysis: A representative lot detected alpha B-crystallin in ELISA applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Inhibition Analysis: A representative lot inhibited the chaperone and anti-apoptotic activities of alpha B-crystallin. (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Immunofluorescence Analysis: A representative lot detected alpha B-crystallin in Immunofluorescence applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Western Blotting Analysis: A representative lot detected alpha B-crystallin in Western Blotting applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).
Biochem/physiol Actions
Clone aB1788G7G6 is a mouse monoclonal antibody that detects Alpha(B)-crystallin. It targets an epitope within 21 amino acids within the N-terminal half.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in mouse retina tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected alpha B-crystallin in mouse retina tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected alpha B-crystallin in mouse retina tissue lysate.
Other Notes
Human, Mouse. Predicted to react with Bovine, Monkey, Rat based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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Certificates of Analysis (COA)
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