Anti-RGMb Antibody, clone 307.9D1

His-taged, Full length mouse Repulsive guidance molecule (RGMb).

Quality Control Testing

Evaluated by Flow Cytometry in J774.2 cells

Flow Cytometry Analysis: 1.0 µg from a representative lot detected RGMb in one million J774.2 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected RGMb in Western Blotting application (Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59).

Flow Cytometry Analysis: A representative lot detected RGMb in Flow Cytometry application (Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59).

Inhibition Assay: A representative lot of this antibody inhibited the interaction of RGMb with PD-L2 and RGMb-BMP and blocked the development of airway hyperreactivity and inflammation. (Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59; Yu, S., et al. (2019). J Allergy Clin Immunol. 144(1):94-108.e11).

Immunocytochemistry Analysis: A representative lot detected RGMb in Immunocytochemistry application (Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59).

ELISA: A representative lot detected RGMb in ELISA application (Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 307.9D1 is a rat monoclonal antibody that specifically detects Repulsive guidance molecule B (RGMb).

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Repulsive guidance molecule B (UniProt: Q7TQ33; also known as RG11-responsive axonal guidance and outgrowth of neurite, DRAGON) is encoded by the Rgmb gene (Gene ID: 68799) in mouse. It is a glycosylphosphatidylinositol-anchored protein, which plays a significant role in various biological processes that contributes to the patterning of the developing nervous system, neuronal ashesion and bone morphogenetic protein (BMP) signaling. RGMb is expressed in multiple tissues, with notable levels in the developing nervous system, adult dorsal root ganglion sensory neurons, spinal cord, and brain. RGMb protein is synthesized with a signal peptide (aa 1-48), propeptide (aa 416-436) which is subsequently cleaved off to produce the mature protein (aa 49-415). Subcellularly, RGMb is localized at the cell membrane, where it interacts with receptors such as neogenin, influencing cell migration and differentiation. Dysregulation of RGMb has been implicated in several diseases, including cancer, where it has been shown to promote tumor progression by enhancing macrophage infiltration and inflammation in tissues such as the kidneys. Studies have highlighted that RGMb′s interaction with TAB1 and TRAF6 can activate downstream signaling pathways, further contributing to its role in disease pathology. Research has demonstrated that programmed death ligand 2 (PD-L2 binds to both PD-1 (inhibits antitumor immunity), and to RGMb, which regulates respiratory immunity, targeting the PD-L2 pathway has therapeutic potential for asthma, cancer, and other immune-mediated disorders. It is suggested that this pathway may provide insights into how to optimally modulate the PD-1 pathway in cancer immunotherapy while minimizing adverse events. This 307.9D1 rat monoclonal antibody has been validated by Flow cytometry, ELISA, Western Blotting, Immunocytochemistry and can be used for inhibition studies.(Ref.: Xiao, Y., et al. (2014). J Exp Med. 211(5):943-59; Yu, S., et al. (2019). J Allergy Clin Immunol. 144(1):94-108.e11).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.