LanC-like protein 2 (UniProt: Q9NS86; also known as Testis-specific adriamycin sensitivity protein) is encoded by the LANCL2 (also known as GPR69B, TASP) gene (Gene ID: 55915) in human. LANC2 is a member of the highly conserved and widely distributed lanthionine synthetase component C-like (LANCL) protein family. It is well expressed in brain and testis and is localized to the plasma membrane, juxta-nuclear vesicles, and the nucleus. The N-terminus of LANCL2 differs from the other LANCL proteins by an N-terminal extension of 17 amino acid residues and an insertion of 19 residues. LANCL2 contains 15 cysteine residues, three of which are conserved in all human LANCL proteins and 8 are conserved between LANCL2 and LANCL1. LANCL2 displays about 75% amino acid homology with LANCL1 protein. LANCL2 contains a phosphatidylinositol phosphate (PIP) binding site at the N-terminus and its myristoylation is essential for its membrane association. LANCL2 is essential for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. Exogenous expression LANCL2 in a sarcoma cell line is shown to reduce the expression of ABCB1 (P-glycoprotein 1) and increase cellular sensitivity to adriamycin. (Ref.: Sturla, L et al. (2009). J. Biol. Chem. 284(41):28045-57; Ladlinger, C et al. (2006). Biochim Biophys Acta. 1758(11):1759-67).

~50 kDa observed; 50.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Clone 392 detected LANCL2 overexpressed in HEK293 cells.

Epitope: unknown

GST-tagged full length human recombinant LANCL2 protein.

Detect LANCL2 using this mouse monoclonal Anti-LANCL2 Antibody, clone 392, Cat. No. MABS1290. It is used in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected LANCL2 in Immunocytochemistry applications (Fresia, C., et. al. (2016). Sci Rep. 6:26658).

Western Blotting Analysis: A representative lot detected LANCL2 in Western Blotting applications (Vigliarolo, T., et. al. (2015). J Biol Chem. 290(21):13042-52; Vigliarolo, T., et. al. (2016). Int J Biochem Cell Biol. 75:99-103).

Research Category
Signaling

Format: Purified

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in lysates from HEK293 cells overexpressing LANCL2.

Western Blotting Analysis: 4 µg/mL of this antibody detected LANCL2 in 10 µg of cell lysate from HEK293 cells overexpressing LANCL2.

Concentration: Please refer to lot specific datasheet.

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