All Photos(2)



Anti-GLUT1 (CT) Antibody, clone 5B12.3

clone 5B12.3, from mouse

Solute carrier family 2 facilitated glucose transporter member 1, Glucose transporter type 1,erythrocyte/brain, GLUT-1, HepG2 glucose transporter

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


5B12.3, monoclonal

species reactivity

rat, human


antibody small pack of 25 μL


immunohistochemistry: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in


storage temp.


Gene Information

human ... SLC2A1(6513)

General description

GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55 kDa and a 45 kDa. The 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.


This antibody recognizes GLUT1 at the C-terminus.


Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT1.


Anti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.
Research Category
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT1 in rat cerebellum and human cerebellum tissues.
Research Sub Category
Developmental Signaling


Evaluated by Western Blot in NGF treated PC-12 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected GLUT1 in 10 µg of NGF treated PC-12 cell lysate.

Target description

~54 kDa observed

Physical form

Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

NGF treated PC-12 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Stanley Andrisse et al.
American journal of physiology. Endocrinology and metabolism, 314(4), E353-E365 (2018-01-20)
Hyperandrogenemia and hyperinsulinemia are believed to play prominent roles in polycystic ovarian syndrome (PCOS). We explored the effects of low-dose dihydrotestosterone (DHT), a model of PCOS, on insulin signaling in metabolic and reproductive tissues in a female mouse model. Insulin
PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
Wang, Z; Song, F; Li, J; Zhang, Y; He, Y; Yang, J; Zhou, H; Zhao, T; Fu, W; Xing, P; Wan et al.
Evidence-Based Complementary and Alternative Medicine : ECAM null
Roberta Battistella et al.
International journal of molecular sciences, 22(21) (2021-11-14)
The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different
Endotoxin-induced inflammation down-regulates L-type amino acid transporter 1 (LAT1) expression at the blood-brain barrier of male rats and mice.
Wittmann, G; Mohacsik, P; Balkhi, MY; Gereben, B; Lechan, RM
Fluids and Barriers of the Cns null
Shikha Prasad et al.
Fluids and barriers of the CNS, 12, 18-18 (2015-07-25)
Diabetes and tobacco smoking are significant public health concerns which have been shown to independently impact the blood-brain barrier (BBB). Since smoking is a risk factor for diabetes and shares some of the common pathological pathways leading to metabolic abnormalities

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service