Mitogen-activated protein kinase 14 (EC 2.7.11.24; UniProt P47811; also known as CRK1, MAP kinase 14, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, p38a, p38alpha, p38MAPK, SAPK2a, Stress-activated protein kinase 2a) is encoded by the Mapk14 (also known as Crk1, Csbp1, Csbp2, Mxi2) gene (Gene ID 26416) in murine species. p38 mitogen-activated protein kinases (MAPKs) constitute a group of stress-activated serine/threonine-specific kinases (SAPKs), including p38α (MAPK14/SAPK2A), p38β (MAPK11/SAPK2B), p38γ (ERK6/MAPK12/SAPK3), and p38δ (MAPK13/SAPK4), that mediate cellular signal transduction and play a vital role in numerous biological processes. p38α and p38β are ubiquitously expressed, while p38γ and p38δ are differentially expressed depending on tissue type. Sequence comparisons show approximately 60% identity within the p38 group. but only 40-45% to the other three MAP kinase family groups, extracellular signal-regulated kinases (ERKs), c-jun N-terminal or stress-activated protein kinases (JNK/SAPK), and ERK/big MAP kinase 1 (BMK1). All four p38 kinases contain a conserved Thr-Gly-Tyr (TGY) dual phosphorylation motif and are activated by dual kinases called MAP kinase kinases (MKKs).

~38 kDa observed.

Full-length murine p38/SAPK2a GST fusion protein.

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-p38 Antibody, clone 2F11 is validated for use in Western Blotting for the detection of SAPK2.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected p38/SAPK2 in 10 µg of mouse (C2C12, NIH/3T3, RAW264), rat (L6, PC12), and human (HEK293, HeLa, HepG2, HUVEC, Jurkat) cell lysates, and in 10 µg of mouse brain homogenate.
Western Blotting Analysis: Representative lots detected p38 in human A549 epidermoid carcinoma cell and WI38-VA13 fibroblast lysates (Song, J.Y., et al. (2010). J. Biol. Chem. 285(12):9067-9076; Song, J.Y., et al. (2009). Cancer Lett. 283(2):135-142).
Western Blotting Analysis: A representative lot detected p38 in mouse embryonic stem cell lysate (Wu, X., et al. (2007). Dev. Dyn. 236(10):2767-2778).

Clone 2F11 recognizes both p38alpha/SAPK2a and p38beta/SAPK2b.

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected p38/SAPK2 in 10 µg of A431 cell lysate.

Concentration: Please refer to lot specific datasheet.

Replaces: 05-454

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