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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
13D3
monoclonal
purified by
using protein G
species reactivity
mouse, human, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
Full-length purified human Proprotein convertase 9 (PCSK9).
Specificity
Clone 13D3 is a mouse monoclonal antibody that specifically detects Proprotein convertase 9 (NARC-1) and its cleaved form in human cells.
Application
Western Blotting Analysis: A representative lot detected NARC-1 (PCSK9) in Western Blotting applications (Zhang, D.W., et. al. (2008). Proc Natl Acad Sci USA. 105(35):13045-50; Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005).
ELISA Analysis: A representative lot detected NARC-1 (PCSK9) in ELISA applications (Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Lakoski, S.G., et. al. (2009). J Clin Endocrinol Metab. 94(7):2537-43).
ELISA Analysis: A representative lot detected NARC-1 (PCSK9) in ELISA applications (Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Lakoski, S.G., et. al. (2009). J Clin Endocrinol Metab. 94(7):2537-43).
Quality
Evaluated by Western Blotting in HepG2 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected NARC-1 (PCSK9) in HepG2 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected NARC-1 (PCSK9) in HepG2 cell lysates.
Physical properties
~75 and 59 kDa observed (proprotein and cleaved form); 74.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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