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Merck
CN

MABS2161

Sigma-Aldrich

Anti-NARC-1 (PCSK9) Antibody, clone 15A6

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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conjugate

unconjugated

antibody form

purified antibody

clone

15A6
monoclonal

purified by

using protein G

species reactivity

mouse, human, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

Full-length purified human Proprotein convertase 9 (PCSK9).
~80 and 60 kDa observed (proprotein and cleaved form); 74.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Western Blotting Analysis: A representative lot detected NARC-1 (PCSK9) in Western Blotting applications (Zhang, D.W., et. al. (2008). Proc Natl Acad Sci USA. 105(35):13045-50; Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

Immunocytochemistry Analysis: A representative lot detected NARC-1 (PCSK9) in Immunocytochemistry applications (Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NARC-1 (PCSK9) in Immunoprecipitation applications (Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

ELISA Analysis: A representative lot detected NARC-1 (PCSK9) in ELISA applications (Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Lakoski, S.G., et. al. (2009). J Clin Endocrinol Metab. 94(7):2537-43).

Biochem/physiol Actions

Clone 15A6 is a mouse monoclonal antibody that specifically detects Proprotein convertase 9 (NARC-1) and its cleaved form in human cells.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in human lung tissue lysates.

Western Blotting Analysis: 4 µg/mL of this antibody detected NARC-1 (PCSK9) in human lung tissue lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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