MABS2185
Anti-NPHP1 Antibody, clone 1M4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1M4, monoclonal
Application:
IF, WB
Citations:
-
conjugate
unconjugated
antibody form
purified antibody
clone
1M4, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunofluorescence: suitable, western blot: suitable
isotype
IgG3κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse
General description
A synthetic polypeptide fused by 16 different NPHP1 peptide sequences predicted to have the highest antigenicity. The sequence was converted to cDNA and the polypeptide was expressed and purified from E. coli.
~92 kDa observed; 83.30 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Immunofluorescence Analysis: A representative lot of this antibody detected NPHP1 in Immunofluorescence applications (Xu, Q., et. al. (2016). Nat Commun. 7:10777).
Biochem/physiol Actions
Clone 1M4 is a mouse monoclonal antibody that specifically detects human Nephrocystin-1 (NPHP1).
Physical form
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human testis tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected NPHP1 in human testis tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected NPHP1 in human testis tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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