MABS2188
Anti-SIRP alpha Antibody, clone SAF10.1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
antibody form
purified antibody
clone
SAF10.1
monoclonal
purified by
using protein G
species reactivity
human, mouse, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
Eukaryotically expressed SIRP-Fc fusion protein consisting of the extracellular domain.
~75 kDa observed; 54.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Western Blotting Analysis: 1 µg/mL from a representative lot detected SIRP alpha in A549 cell lysate.
Western Blotting Analysis: A represetntative lot detected SIRP alpha in Western Blotting applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).
ELISA Analysis: A represetntative lot detected SIRP alpha in ELISA applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).
Western Blotting Analysis: A represetntative lot detected SIRP alpha in Western Blotting applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).
ELISA Analysis: A represetntative lot detected SIRP alpha in ELISA applications (Lee, W.Y., et. al. (2007). J. Immunol. 179(11); 7741-50).
Biochem/physiol Actions
Clone SAF10.1 is a mouse monoclonal antibody that detects human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRP alpha). It targets an epitope in the membrane most distal domain (D1).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SIRP alpha in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SIRP alpha in U20S cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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