Merck
CN
All Photos(2)

Documents

MABS522

Sigma-Aldrich

Anti-PIKFYVE Antibody, clone R159.4.3C9

clone R159.4.3C9, from mouse

Sign Into View Organizational & Contract Pricing

Select a Size

Change View
Synonym(s):
1-phosphatidylinositol 3-phosphate 5-kinase, Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

R159.4.3C9, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4500758P0054MABC195
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

R159.4.3C9, monoclonal

clone

polyclonal

clone

polyclonal

clone

7F11.1, monoclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

human

species reactivity

human

technique(s)

western blot: suitable

technique(s)

ELISA: 1:5000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

technique(s)

western blot: 2-4 μg/mL using HEK-293T cells expressing human PIP5K3.

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG2aκ

isotype

-

isotype

-

isotype

IgG2aκ

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.

Specificity

Expected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).

Immunogen

AviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.
Epitope: Internal (N-terminal half).

Application

Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PIKFYVE in 10 µg of HepG2 cell lysate.

Quality

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PIKFYVE in 20 µg of HL-60 cell lysate.

Target description

~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Sindhu Carmen Sivakumaren et al.
Cell chemical biology, 27(5), 525-537 (2020-03-05)
The PI5P4Ks have been demonstrated to be important for cancer cell proliferation and other diseases. However, the therapeutic potential of targeting these kinases is understudied due to a lack of potent, specific small molecules available. Here, we present the discovery

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service