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MABT100

Anti-Girdin Antibody, CT, clone 10E6.1

Name: Anti-Girdin Antibody, CT, clone 10E6.1
Brand: MM

clone 10E6.1, from mouse

Synonym(s):

Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

10E6.1, monoclonal

Application:

ICC, IHC, IP, WB

Citations:

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Properties

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10E6.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001129069

UniProt accession no.

Q3V6T2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CCDC88A(55704)

Description

General description

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

~216 kDa observed

Immunogen

Recombinant protein corresponding to human Girdin.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.

This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c