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MABT100

Anti-Girdin Antibody, CT, clone 10E6.1

clone 10E6.1, from mouse

Synonym(s):

Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
10E6.1, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
3
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10E6.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001129069

UniProt accession no.

Q3V6T2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CCDC88A(55704)

General description

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
~216 kDa observed

Immunogen

Recombinant protein corresponding to human Girdin.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.
This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Related Content

Research Focus - Volume 4 - 2012

"New! PI 3-Kinase/Akt Signaling Research Tools. PI 3-kinases are ubiquitous, heterodimeric lipid kinases that regulate cell growth, motility, proliferation, survival and other processes. These dual-specificity enzymes phosphorylate phosphoinositides. The family of eight PI 3-kinases is divided into four classes, which differ in their substrate selectivity and regulation."

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Oncotarget, 10(30), 2869-2886 (2019-05-14)
We report the use of small interfering RNAs (siRNAs) against ARHGEF4, CCDC88A, LAMTOR2, mTOR, NUP85, and WASF2 and folic acid (FA)-modified polyethylene glycol (PEG)-chitosan oligosaccharide lactate (COL) nanoparticles for targeting, imaging, delivery, gene silencing, and inhibition of invasiveness and metastasis
Aki Tanouchi et al.
Journal of experimental & clinical cancer research : CR, 35(1), 190-190 (2016-12-07)
Coiled-Coil Domain Containing 88A (CCDC88A) was identified as a substrate of the serine/threonine kinase Akt that is capable of binding to the actin cytoskeleton. The aim of this study was to investigate the potential role of CCDC88A in the migration
Pradipta Ghosh et al.
Current protocols in chemical biology, 8(4), 265-298 (2016-12-08)
Canonical signal transduction via heterotrimeric G proteins is spatiotemporally restricted, i.e., triggered exclusively at the plasma membrane, only by agonist activation of G protein-coupled receptors via a finite process that is terminated within a few hundred milliseconds. Recently, a rapidly

Global Trade Item Number

SKUGTIN
MABT10004053252643422