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MSIPS4W10

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

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eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

400

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

ELISPOT: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

Related Categories

General description

The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.

Application

MultiScreen® HTS IP Filter Plate, 0.45 μm, white, sterile has been used in enzyme-linked immunospot (ELISpot) assay using:
  • dsDNA-producing plasma cells
  • memory B cells and bone marrow localized long-lived plasma cells
  • draining lymph node and spleen cells

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain

Articles

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Protocols

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Related Content

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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