NB21
Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1)
liquid, clone NSF-1, Calbiochem®
Synonym(s):
Anti-NSF
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
NSF-1, monoclonal
form
liquid
contains
3 mM sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
This product has been discontinued.
Recognizes the ~76 kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~76 kDa NSF-1 protein.
Immunogen
Human
recombinant, human N-ethylmaleimide-senstive factor
Application
ELISA (1:2000)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human or rat fibroblasts, MDBK cells, or PC12 cells
Human or rat fibroblasts, MDBK cells, or PC12 cells
Other Notes
Sutton, R.B., et al. 1998. Nature395, 347.
Lemons, P.P., et al. 1997. Blood90, 1490.
Edelmann, L., et al. 1995. EMBO J.14, 224.
Südhof, T.C. 1995. Nature375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol.126, 945.
Lemons, P.P., et al. 1997. Blood90, 1490.
Edelmann, L., et al. 1995. EMBO J.14, 224.
Südhof, T.C. 1995. Nature375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol.126, 945.
This antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10-13 - German Storage Class 10 to 13
Regulatory Information
新产品
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mark Boltengagen et al.
Nucleic acids research, 44(5), 2145-2159 (2015-11-21)
The incorporation of CENP-A into centromeric chromatin is an essential prerequisite for kinetochore formation. Yet, the molecular mechanisms governing this process are surprisingly divergent in different organisms. While CENP-A loading mechanisms have been studied in some detail in mammals, there
Dakshnamurthy Selvakumar et al.
The Biochemical journal, 474(1), 79-104 (2016-11-09)
Dopamine receptors regulate exocytosis via protein-protein interactions (PPIs) as well as via adenylyl cyclase transduction pathways. Evidence has been obtained for PPIs in inner ear hair cells coupling D1A to soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE)-related proteins snapin
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Sang H Lee et al.
Nature communications, 9(1), 3434-3434 (2018-08-26)
Secreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting synapse elimination in central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses and acts as a Wnt inhibitor.