NB37
Anti-Golgi Apparatus Mouse mAb (AE-6)
liquid, clone AE-6, Calbiochem®
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
AE-6
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells. Recognizes the golgi apparatus protein.
Recognizes the Golgi apparatus in A431 and HeLa cells.
Immunogen
Human
the Golgi fraction of human cells
Application
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1 µg/ml)
Immunofluorescence (1 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Positive Control
A-431 or HeLa cells
A-431 or HeLa cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Gomord, V., et al. 1999. Biochimie81, 607.
Burkhardt, J.K. 1998. Biochim. Biophys. Acta1404, 113.
Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta1404, 139.
Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol.10, 477.
Keenan, T.W., 1998. Histochem. Cell Biol.109, 505.
La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta1404, 195.
Munro, S., 1998. Trends Cell Biol.8, 11.
Pelham, H. R., 1998. Trends Cell Biol.8, 45.
Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol.10, 4993.
Burkhardt, J.K. 1998. Biochim. Biophys. Acta1404, 113.
Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta1404, 139.
Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol.10, 477.
Keenan, T.W., 1998. Histochem. Cell Biol.109, 505.
La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta1404, 195.
Munro, S., 1998. Trends Cell Biol.8, 11.
Pelham, H. R., 1998. Trends Cell Biol.8, 45.
Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol.10, 4993.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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V Gomord et al.
Biochimie, 81(6), 607-618 (1999-08-05)
Protein transport along the secretory pathway is supported by a noria of vesicles that bud and fuse, load and unload their cargo from one compartment into the other. However, despite this constant flow-through of proteins and lipids the various compartments
Biochemistry of the Golgi apparatus.
T W Keenan
Histochemistry and cell biology, 109(5-6), 505-516 (1998-07-29)
R Le Borgne et al.
Biochimica et biophysica acta, 1404(1-2), 195-209 (1998-08-26)
The trans-Golgi network (TGN) is the last station of the secretory pathway where soluble and membrane proteins are sorted for subsequent transport to endocytic compartments. This pathway is primarily followed by two distinct but related mannose 6-phosphate receptors which exhibit
J K Burkhardt
Biochimica et biophysica acta, 1404(1-2), 113-126 (1998-08-26)
The intimate association between the Golgi complex and the microtubule cytoskeleton plays an important role in Golgi structure and function. Recent evidence indicates that the dynamic flow of material from the ER to the Golgi is crucial to maintaining the
N Cabrera-Poch et al.
Biochimica et biophysica acta, 1404(1-2), 139-151 (1998-08-26)
The creation and propagation of the intricate Golgi architecture during the cell cycle poses a fascinating problem for biologists. Similar to the inheritance process for nuclear DNA, the inheritance of the Golgi apparatus consists of biogenesis (replication) and partitioning (mitosis/meiosis)
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