OP64F
Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate
liquid, clone EA10, Calbiochem®
Synonym(s):
Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
Product Name
Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate, liquid, clone EA10, Calbiochem®
biological source
mouse
antibody product type
primary antibodies
clone
EA10, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
Quality Level
Analysis Note
Negative Control
Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue
Any cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue
Application
Flow Cytometry (2 µg/ml)
Frozen Sections (5 µg/ml)
Immunoblotting (use Cat. No. OP64)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP64)
Paraffin Sections (5 µg/ml, see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (use Cat. No. OP64)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP64)
Paraffin Sections (5 µg/ml, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
This product has been discontinued.
Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
Purified mouse monoclonal antibody generated by immunizing F1 mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~21 kDa p21WAF1 protein.
Immunogen
Epitope: within amino acids 58-77 of human p21WAF1
Human
full-length, recombinant, human p21WAF1
Other Notes
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates p21WAF1 expression. Serum stimulation of quiescent cells will give low level p21WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can also be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. For staining paraffin sections, no pretreatment is usually needed, but some tissues may stain better after pretreating with heat or pressure cooker. In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. For immunoprecipitation and immunoblotting use Cat. No. OP64. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Regulatory Information
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