OP79
Anti-p21WAF1 (Ab-6) Mouse mAb (22)
liquid, clone 22, Calbiochem®
Synonym(s):
Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
biological source
mouse
antibody product type
primary antibodies
clone
22, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
This product has been discontinued.
Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.
Purified mouse monoclonal antibody, generated by immunizing BALB/c x C57B1/6 F1 mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~21 kDa p21WAF1 protein.
Immunogen
Epitope: within amino acids 20-40 of human p21WAF1
recombinant, mouse p21WAF1
Application
Frozen Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Induced rat or mouse embryo fibroblasts
Induced rat or mouse embryo fibroblasts
Other Notes
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence detection methods. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Regulatory Information
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K E King et al.
Carcinogenesis, 27(1), 53-63 (2005-08-06)
p63 is critical for squamous development and exists as multiple isotypes of two subclasses, TA and DeltaN. DeltaNp63 isotypes can antagonize transcription by TAp63 and p53, and are highly expressed in squamous cell cancers. Using mouse keratinocytes as a biological
Matthew Jennis et al.
Genes & development, 30(8), 918-930 (2016-04-02)
A nonsynonymous single-nucleotide polymorphism at codon 47 in TP53 exists in African-descent populations (P47S, rs1800371; referred to here as S47). Here we report that, in human cell lines and a mouse model, the S47 variant exhibits a modest decrease in
Gregory Azzam et al.
PloS one, 8(9), e74297-e74297 (2013-09-11)
The p53 tumor suppressor gene has a common polymorphism at codon 72 that alters its function. We previously reported that the proline 72 polymorphic variant of p53 (P72) demonstrates increased ability to transactivate a subset of genes, relative to arginine
Che-Pei Kung et al.
Cancer biology & therapy, 18(7), 484-491 (2017-05-06)
The TP53 gene is distinguished as the most frequently mutated gene in cancer. Unlike most cancer-relevant genes, the TP53 gene is also distinguished by the existence of coding region polymorphisms that alter p53 sequence, and in some cases, also alter
Chih-Yen Wang et al.
Frontiers in molecular neuroscience, 11, 4-4 (2018-02-09)
B-cell CLL/lymphoma 11B (Bcl11b) - a C2H2 zinc finger transcriptional factor - is known to regulate neuronal differentiation and function in the development of the central nervous system (CNS). Although its expression is reduced during oligodendrocyte (OLG) differentiation, its biological
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