PC10L
Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb
lyophilized, Calbiochem®
Product Name
Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb, lyophilized, Calbiochem®
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
Quality Level
Analysis Note
Negative Control
SW480 cells
SW480 cells
Positive Control
HS578T cells or purified recombinant K-Ras Asp¹² protein
HS578T cells or purified recombinant K-Ras Asp¹² protein
Application
Immunoblotting (1-5 µg/ml)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified rabbit polyclonal antibody. Recognizes the ~21 kDa H-, K-, and N-Ras proteins.
Recognizes the ~21 kDa H-, K-, and N-Ras proteins containing aspartic acid at position 12.
Immunogen
a synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
Other Notes
Bos, J.L., et al. 1988. Mutation Res.195, 255.
Furth, M.E., et al. 1987. Oncogene1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1990 Nature287, 686.
Furth, M.E., et al. 1987. Oncogene1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1990 Nature287, 686.
Does not react with rasGly-12 or rasVal-12 p21′s. Antibody should be titrated for optimal results in individual systems.
Physical form
Lyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
Preparation Note
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10-13 - German Storage Class 10 to 13
Regulatory Information
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Daniela S Bassères et al.
Cancer research, 70(9), 3537-3546 (2010-04-22)
K-Ras-induced lung cancer is a very common disease, for which there are currently no effective therapies. Because therapy directly targeting the activity of oncogenic Ras has been unsuccessful, a different approach for novel therapy design is to identify critical Ras
Chun-Mei Hu et al.
Cell metabolism, 29(6), 1334-1349 (2019-03-12)
KRAS mutations are the earliest events found in approximately 90% of pancreatic ductal adenocarcinomas (PDACs). However, little is known as to why KRAS mutations preferentially occur in PDACs and what processes/factors generate these mutations. While abnormal carbohydrate metabolism is associated
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