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Sigma-Aldrich

Anti-FGF, Basic (Ab-2) (40-63) Rabbit pAb

liquid, Calbiochem®

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Synonym(s):
Anti-Fibroblast Growth Factor, Basic, Anti-FGF-2, Anti-Heparin Binding Growth Factor, Anti-HBGF-1

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGF2(2247)

General description

Anti-FGF, Basic (Ab-2) (40-63), rabbit polyclonal, recognizes the ~18 kDa bFGF protein in fetal liver and kidney. It is validated for Western blotting an immunohistochemistry (paraffin sections).
Purified rabbit polyclonal antibody (see application references). Recognizes the ~18 kDa bFGF protein.
Recognizes the ~18 kDa bFGF protein in fetal liver and kidney tissue.

Immunogen

Human
a synthetic peptide (GFFLRIHPDGRVDGVREKSDPHIK) corresponding to amino acids 40-63 of human bFGF

Application

Immunoblotting (1-5 µg/ml)

Paraffin Sections (1-5 µg/ml, no pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Fetal liver or kidney tissue

Other Notes

Antibody should be titrated for optimal results in individual systems.
Burton, P.B.J., et al. 1993. Int. J. Exp. Path.74, 87.
Fiege, J.J. and Baird, A. 1989. Proc. Natl. Acad. Sci.86, 3174.
Florkiewicz, R.Z. and Sommer, A. 1989. Proc. Natl. Acad. Sci.86, 3978.
Rifkin, D.B. and Moscatelli, D. 1989. J. Cell Biol.109, 1.
Baird, A., et al. 1988. Proc. Natl. Acad. Sci.85, 2324.
Chodak, G.W., et al. 1988. Cancer Res. 48, 2083.
Sommer, A., et al. 1987. Res. Comm.144, 543.
Abraham, J.A., et al.1986. EMBO J.5, 2523.
Esch, F., et al.1985. Proc. Natl. Acad. Sci.82, 6507.
Knighton, D., et al. 1977. Br. J. Cancer35, 347.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

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Frederique M D Tholozan et al.
Molecular biology of the cell, 18(11), 4222-4231 (2007-08-19)
The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary
Monika Damek-Poprawa et al.
Oral surgery, oral medicine, oral pathology and oral radiology, 115(2), 201-211 (2012-12-21)
Osteoradionecrosis (ORN) is common in the jaws after radiotherapy. We hypothesized that the mandible is more susceptible to ORN than the tibia, based on site disparity in hypoxic, hypocellular, and hypovascular tissue breakdown. Twelve rats received 50 Gy irradiation to

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