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PC322L

Sigma-Aldrich

Anti-VEGFR1 Goat pAb

lyophilized, Calbiochem®

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Synonym(s):
Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor

biological source

goat

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLT1(2321)

General description

Immunoaffinity purified goat polyclonal antibody. Recognizes the VEGF receptor 1 protein.
Recognizes VEGF receptor 1.
This Anti-VEGFR1 Goat pAb is validated for use in Competition Studies, ELISA, Immunoblotting, Paraffin Sections for the detection of VEGFR1.

Immunogen

Human
recombinant, human VEGFR1 (extracellular domain)/Fc chimera

Application

Competition Studies (see comments)

ELISA (0.5-1 µg/ml)

Immunoblotting (0.1-0.2 µg/ml)

Paraffin Sections (5-15 µg/ml)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from a sterile solution in PBS, 5% trehalose.

Reconstitution

Reconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Endothelial cells

Other Notes

Soker, S., et al. 1998. Cell92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS.90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303.
Brown, L., et al. 1992. Kidney International42, 1457.
This antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Meghan T Logun et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(11), 11973-11992 (2019-08-10)
Invasive spread of glioblastoma (GBM) is linked to changes in chondroitin sulfate (CS) proteoglycan (CSPG)-associated sulfated glycosaminoglycans (GAGs) that are selectively up-regulated in the tumor microenvironment (TME). We hypothesized that inhibiting CS-GAG signaling in the TME would stem GBM invasion.

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