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PC635

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

Name: PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Nibrin (pSer³⁴³) Rabbit pAb

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human

Application:

—

Citations:

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Properties

biological source

rabbit

Quality Segment

100

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µL/500 µg cell extract))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer343)

Gene Information

human ... NBN(4683)

Description

General description

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.

This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

Immunogen

Human

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

Application

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

Physical form

Serum diluted in PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
γ-irradiated lymphoblastoid cells

Other Notes

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.

This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c