PK1109
PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb
liquid, Calbiochem®
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
mouse, rat, chicken, human, Xenopus
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr416)
Gene Information
human ... SRC(6714)
General description
Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr416.
Recognizes the ~60 kDa Src protein phosphorylated at Tyr416 in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.
This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src
Application
Immunoblotting (1:1000)
Physical form
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
Analysis Note
Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma
Other Notes
May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Thomas, S.M. and Brugge, J.S. 1997. Annu. Rev. Cell Dev. Biol.13, 513.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jeremy Roy et al.
Nature communications, 7, 10666-10666 (2016-02-13)
Epithelial cells are generally considered to be static relative to their neighbours. Basal cells in pseudostratified epithelia display a single long cytoplasmic process that can cross the tight junction barrier to reach the lumen. Using in vivo microscopy to visualize
Sevgi Irtegun et al.
PloS one, 8(7), e71035-e71035 (2013-08-08)
c-Src kinase activity is regulated by phosphorylation of Y527 and Y416. Y527 phosphorylation stabilizes a closed conformation, which suppresses kinase activity towards substrates, whereas phosphorylation at Y416 promotes an elevated kinase activity by stabilizing the activation loop in a manner
L P Stabile et al.
Oral oncology, 69, 38-45 (2017-06-01)
Src family kinase (SFK) activation circumvents epidermal growth factor receptor (EGFR) targeting in head and neck squamous cell carcinoma (HNSCC); dual SFK-EGFR targeting could overcome cetuximab resistance. We conducted a Simon two-stage, phase II trial of the SFK inhibitor, dasatinib
Sandra Barisic et al.
Biochemical pharmacology, 80(4), 439-447 (2010-05-11)
Activation status of Tyr-kinase Src as well as of the transcription factor NFkappaB is a decisive criterion for the onset of cancer and in conveying radio-resistance. While the activation status of Src is Tyr phosphorylation-dependent, NFkappaB activation requires Ser phosphorylation
Athanassios Argiris et al.
Investigational new drugs, 30(4), 1575-1584 (2011-09-02)
Combined inhibition of epidermal growth factor receptor (EGFR) and Src family kinases (SFK) may lead to improved therapeutic effects. We evaluated the combination of dasatinib, an inhibitor of SFK and other kinases, and cetuximab, an anti-EGFR monoclonal antibody. Patients with
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