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PRCYT2MAG40K

Millipore

MILLIPLEX® Non-Human Primate Cytokine/Chemokine Panel II - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

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eCl@ss:
32161000

Quality Level

200

species reactivity

nonhuman primates

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-109%
(Overnight Incubation)
standard curve range: 1-1,500 pg/mL
(IL-17A)
standard curve range: 1-750 pg/mL
(RANTES/CCL5 (tissue culture))
standard curve range: 10-10,000 pg/mL
(sCD137, IL-2, IP-10/CXCL10)
standard curve range: 146-150,000 pg/mL
(IL-4)
standard curve range: 15-15,000 pg/mL
(IL-21, IL-31)
standard curve range: 20-20,000 pg/mL
(FGF-2, IL-33)
standard curve range: 24-25,000 pg/mL
(IL-23)
standard curve range: 29-30,000 pg/mL
(IL-1α, IL-28A)
standard curve range: 293-300,000 pg/mL
(IL-17E/IL-25)
standard curve range: 3-3,000 pg/mL
(Eotaxin/CCL11, IL-6)
standard curve range: 4-4,000 pg/mL
(Granzyme B)
standard curve range: 7-7,000 pg/mL
(MIP-3α/CCL20)
standard curve range: 73-75,000 pg/mL
(IL-16)
standard curve range: 78-80,000 pg/mL
(Granzyme A)
standard curve range: 8-8,000 pg/mL
(sFasL)
standard curve range: 98-100,000 pg/mL
(Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

The MILLIPLEX® Non-Human Primate Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of any or all of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA
  • Recommended Sample type: Serum, plasma, or cell culture supernatant
  • Recommended Sample dilution: 1:2 diluted plasma or serum
  • NOTE: RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs
  • Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Components

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized

Sample Plate, 96 well format, 1 plate

2 Foil plate sealers

Assay Buffer, 2 bottles, 30 mL

Bead Diluent, 1 bottle, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Other Notes

Please contact Technical Service for linearity of dilution.
Please note: Sample Type must be selected when configuring this kit. RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes for serum/plasma.
Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Information

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Dapeng Li et al.
Cell, 184(16), 4203-4219 (2021-07-10)
SARS-CoV-2-neutralizing antibodies (NAbs) protect against COVID-19. A concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated NAbs against the receptor-binding domain (RBD) or the N-terminal domain (NTD) of SARS-CoV-2 spike from individuals with acute or convalescent
François Enchéry et al.
Communications biology, 4(1), 1292-1292 (2021-11-18)
Nipah virus (NiV) is a highly pathogenic emerging bat-borne Henipavirus that has caused numerous outbreaks with public health concerns. It is able to inhibit the host innate immune response. Since the NF-κB pathway plays a crucial role in the innate

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