SCC279
P493-6 Human Lymphoblastoid Cell Line
Synonym(s):
P-493-6, p493-6
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About This Item
NACRES:
NA.45
UNSPSC Code:
41106514
packaging
vial of ≥1X10⁶ cells/vial
technique(s)
cell culture | mammalian: suitable
General description
The proto-oncogene c-myc is an activator of cell proliferation, promoting cell cycle progression, and is thus significant factor in many cancers. Burkitt’s lymphoma is a rare cancer of human B cells associated with Epstein-Barr virus (EBV) that is the most rapidly growing human tumor, due to transcriptional activation of c-myc by chromosomal translocation (1). Because of its highly aggressive nature, Burkitt’s lymphoma has a high mortality rate if left untreated. Cellular models of c-myc-activated lymphomas are essential for understanding mechanisms of proliferation and for developing treatments for this disease. The P493-6 Human Lymphoblastoid Cell Line expresses c-myc under control of a tetracycline-inducible promoter (2). P493-6 cells constitutively express c-myc when cultured without tetracycline. Addition of tetracycline switches off c-myc expression and arrests cells in G0/G1 phase. P493-6 cells demonstrate the morphology and surface antigen expression typical of Burkitt lymphoma cells, including CD10 and CD38 (3).
Source:
The P493-6 cell line was established from a clone of the EREB2-5 EBV-positive lymphoblastoid cell line stably transfected with a c-myc-tet plasmid construct (2).
References:
1. Lancet. 2012; 379(9822): 1234-1244.
2. Int J Cancer. 2000; 87(6): 787-793.
3. Int J Cancer. 2001; 93(6): 810-816
Source:
The P493-6 cell line was established from a clone of the EREB2-5 EBV-positive lymphoblastoid cell line stably transfected with a c-myc-tet plasmid construct (2).
References:
1. Lancet. 2012; 379(9822): 1234-1244.
2. Int J Cancer. 2000; 87(6): 787-793.
3. Int J Cancer. 2001; 93(6): 810-816
Application
- Each vial contains ≥ 1X10⁶ viable cells.
- Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are negative for mycoplasma contamination.
- Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Biochem/physiol Actions
Cancer Cells
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Disclaimer
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled.Click here for Enduser Declaration (EUD) Form.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
高风险级别生物产品-Merck
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