SCC572
3dGRO® Human iPSC Derived Liver Organoid Progenitors
Synonym(s):
Hepatic PDOs, Hepatocyte Organoids, Human Hepatic Organoids, Human Liver PDOs
biological source
human
Quality Level
packaging
vial of ≥1500 vial (viable cells per vial)
manufacturer/tradename
Millipore
technique(s)
cell culture | stem cell: suitable
shipped in
liquid nitrogen
storage temp.
−196°C
General description
3dGRO® Human iPSC-derived Liver Progenitor Organoids are derived from integration-free human induced pluripotent stem cells (iPSC, Cat No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPSC cells were differentiated using a multi-step process from definitive endoderm to hepatic endoderm and eventually into liver progenitor organoids. These liver progenitor organoids are polarized self-organizing, 3D structures that can be expanded in long-term culture and cryo-preservable. These liver progenitor organoids show persistent growth and expansion post cryo-preservation. 3dGRO® human iPSC-derived liver progenitor organoids can be propagated long-term by passaging every 8-10 days using 3dGRO® Human Liver Progenitor Organoid Expansion Media (Cat. no. SCM313).
The 3dGRO® Human iPSC Derived Liver Organoid Progenitor express key liver progenitor markers HNF4a, SOX17, FOXA2 and SOX9, and showed proper apical and basal-lateral polarization marked by E-cadherin and ZO1 expression. 3dGRO® Human iPSC-derived Liver Progenitor Organoids can be differentiated into mature liver organoids containing functional hepatocytes and cholangiocytes when cultured in differentiation media for 14 days.
The 3dGRO® Human iPSC Derived Liver Organoid Progenitor express key liver progenitor markers HNF4a, SOX17, FOXA2 and SOX9, and showed proper apical and basal-lateral polarization marked by E-cadherin and ZO1 expression. 3dGRO® Human iPSC-derived Liver Progenitor Organoids can be differentiated into mature liver organoids containing functional hepatocytes and cholangiocytes when cultured in differentiation media for 14 days.
Application
- Viability: >1500 viable organoids/vial
- Organoid Growth: Pass
- Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Mycoplasma Contamination: Negative
- STR Profile: Pass
Features and Benefits
Cryopreserved iPSC derived human liver organoids (PDOs)
Preparation Note
Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
3dGRO® is a trademark of Merck KGaA, Darmstadt, Germany.
Legal Information
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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Certificates of Analysis (COA)
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