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Merck
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SF-CMTI-2

ESGRO® Complete Adapted C57/BL6 Mouse ES Cell Line

Mouse

Synonym(s):

BRUCE-4 ES Cells, BRUCE-4 ES cells, CMTI-2 Mouse ES Cells

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About This Item

UNSPSC Code:
41106509
NACRES:
NA.52
eCl@ss:
32011203
Biological source:
mouse
Manufacturer/tradename:
Specialty Media

Key Documents

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Product Name

ESGRO® Complete Adapted C57/BL6 Mouse ES Cell Line, The Embryonic Stem Cell Lines derived from murine strain C57/BL6J are selected for high targeting efficiency & proven germ line transmission.

biological source

mouse

manufacturer/tradename

Specialty Media

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Embryonic Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 12. This ES cell line has been pre-adapted to serum-free and feeder-free cell culture conditions and is intended for use with the ESGRO Complete cell culture system.

Packaging

2.5x106 ea

Preparation Note

Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.

Legal Information

ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Meifang Li et al.
Chinese medicine, 15, 83-83 (2020-08-11)
Huyang Yangkun Formula (HYYKF) was developed based on theory of traditional Chinese medicine as well as clinical experience and used to improve ovarian function of premature ovarian insufficiency (POI) patients. Transplantation of embryonic stem cells (ESCs) has great potential in
Jixue Li et al.
The CRISPR journal, 3(6), 470-486 (2020-11-05)
We describe a protocol for the precise integration of exogenous DNA into user-defined genomic loci in cultured cells. This strategy first introduces a promoter and a lox site to a specific location via a Cas9-induced double-strand break. Second, a gene
Baisakhi Mondal et al.
eLife, 9 (2020-04-17)
The mitotic deacetylase complex (MiDAC) is a recently identified histone deacetylase (HDAC) complex. While other HDAC complexes have been implicated in neurogenesis, the physiological role of MiDAC remains unknown. Here, we show that MiDAC constitutes an important regulator of neural

Related Content

ESGRO Complete Products for Mouse Embryonic Stem Cell Culture in Serum-Free and Feeder-Free Conditions
Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

Derivation of Mouse Embryonic Stem Cells in Serum-Free and Feeder-Free Conditions
Data_Sheet-SFCMTI2
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