Skip to Content
Merck
CN

SNAP2MINI

Millipore

SNAP id® 2.0 Protein Detection System-Mini

7.5 x 8.4 cm, Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane

Synonym(s):

Protein detection kit

Sign Into View Organizational & Contract Pricing

Select a Size


About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SNAP id® 2.0 Protein Detection System-Mini (7.5 x 8.4 cm), Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..

manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents
for use with Luminata Western HRP Substrates
for use with Nitrocellulose
for use with PVDF (Immobilon membranes)
for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01)
for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01)
for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001)
for use with commercially available detection reagents

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

storage temp.

room temp

General description

The newly developed SNAP id 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on western blots. With this unique vacuum-driven system, the length of time required for immunodetection is greatly reduced. The classical western blotting time frame of 4- 24 hours is now accomplished in 30 minutes with no quality and signal compromisation. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP id 2.0 system. SNAP id 2.0 Mini Blot Holder accepts up to 7.5 × 8.4 cm blots.

Application

SNAP id 2.0 Protein Detection System-Mini (7.5 x 8.4 cm) has been used in western blot analysis.

Features and Benefits

SNAP id 2.0 Protein Detection System Features:
  • Processing of up to four blots at a time on a vacuum base with two individually controlled sides
  • Comprises of three removable blot holding frame sizes: MultiBlot, Mini, and Midi, to accommodate different blot sizes
  • Disposable blot holders, sized for MultiBlot, Mini, and Midi frames
  • Blot spacer required with first-generation SNAP id system is now integrated into the new blot holder
  • Extended and off-line blot processing options: frames have lids and can be removed from the base for extended incubation (one hour to overnight), incubation in a shaker, or incubation at 4 °C
  • Stackable frames so multiple blots can be processed at the same time;30-minute immunodetection with uniform signal across the blot
  • Greater than 80% antibody recovery using the SNAP id 2.0 Antibody Collection Trays
  • Compatible with nitrocellulose and polyvinylidene fluoride (PVDF) membranes
  • Works with the most blocking buffers and visualization methodologies (e.g. chemiluminescence, fluorescence, or colorimetric)

Packaging

This system includes the SNAP id® 2.0 Base, two Mini Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start Guide

Other Notes

Replaces: WBAVDBASE
The SNAP id® 2.0 Protein Detection System is intended for research use only. It is not for use in diagnostic procedures.

Legal Information

SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Stephen A Fleming et al.
Nutritional neuroscience, 22(7), 499-512 (2017-12-19)
Previous studies have shown that dietary prebiotics have the potential to improve memory, alter social behavior, and reduce anxiety-like behaviors in rodents. The present research sought to expand upon such results and describe the effects of feeding prebiotics early in

Articles

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Protocols

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

Related Content

我们明白。西部片的污点可能会让人头疼,我们当然也有过这样的经历。因此,请与我们分享您的丑陋污点,并获得一份惊喜!虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service