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ST1049

Sigma-Aldrich

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

liquid, clone MCP231, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MCP231, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, yeast, rat, rabbit, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PSMA1(5682)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.
Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

Immunogen

Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
dinitrophenylated proteasomes

Application


Immunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)

Warning

Toxicity: Highly Toxic (H)

Analysis Note

Positive Control
Human placental tissue

Other Notes

Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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